【摘要】 目的　研究低频超声对 Molt- 4肿瘤细胞的细胞周期和细胞凋亡的影响 ,并评价低频超声在肿瘤治疗中的应用价值。方法　应用流式细胞术研究低频超声 (2 0 k Hz,0 .3W/ cm2 )持续辐照 Molt- 4肿瘤细胞 1min后的细胞凋亡率和细胞周期分布的变化。结果　对照组和辐照组细胞凋亡率分别为 (2 .5 6± 0 .6 1) %和 (11.0 6± 1.99) % ,G0 / G1 期百分比分别为 (5 9.78± 3.4 9) %和(79.97± 3.78) % ,S+G2 / M期百分比分别为 (40 .2 2± 3.4 9) %和 (2 0 .0 3± 3.78) % (P<0 .0 1)。凋亡增殖比 (APR)上调。结论　低频超声在体外能够诱导 Molt- 4肿瘤细胞凋亡和抑制肿瘤细胞增殖 ,提示低频超声在肿瘤治疗的临床应用中具有较好潜力。更多还原

【Abstract】 Objective To observe the bioeffects of Molt 4 cells treated with low frequency ultrasound and to study the feasibility of cancer treatment by low frequency ultrasound.Methods With flowcytometry we analysed the percentage changes of apoptosis and the distributional percentage of cell cycle after ultrasonic exposure(20 kHz,0 3 W/cm 2,1 min).Results The percentage of apoptosis in the control group( n =8) and interventional group( n =8) was 2 56±0 61% and 11 06±1 99% respectively The percentage of G0/G1 phase in cell cycle was 59 78±3 49% and 79 97±3 78% respectively The percentage of S+G2/M phase was 40 22±3 49% and 20 03±3 78% respectively (all P <0 01) The ratio of apoptosis to proliferation was up regulated.Conclusions Low frequency ultrasound may be an adjuvant for cancer therapy with its capacitiy of inducing cancer cells apoptosis and inhibiting their proliferation in vitro.更多还原