【摘要】 目的 :研究胃肠道恶性肿瘤患者纤溶分子标志物血浆含量、mRNA水平的变化 ,初步探讨两者与肿瘤浸润、播散的关系及其临床检测意义。方法 :采用逆转录、实时定量PCR技术检测 2 3例胃癌、17例肠癌患者组织中组织型纤溶酶原激活剂 (t PA)、尿激酶型纤溶酶原激活剂 (u PA)mRNA水平 ;用ELISA法同步检测患者血浆纤溶分子标志物含量 ,包括t PA、u PA、尿激酶型纤溶酶原激活剂受体 (u PAR)、纤溶酶 抗纤溶酶复合物 (PAP)等。结果 :胃癌、肠癌组患者血浆u PA、u PAR、PAP蛋白含量均较正常对照明显升高 ,其中 ,有局部浸润、淋巴结转移、远处脏器转移者u PA升高更为显著 ;t PA含量与正常对照无显著差异。u PAmRNA在两种肿瘤细胞表达均显著增高 ,而t PAmRNA则减少。结论 :纤溶功能亢进是胃肠道恶性肿瘤细胞易播散、浸润的主要原因之一。t PAmRNA水平的升高可能为组织分化较好的特征更多还原

【Abstract】 Purpose:To study the changes of t-PA ?u-PA expression and fibrinolysis molecular mark ers in patients with gastric or intestinal cancer in order to elucidate their cl inical significance. Methods:The plasma levels of t-PA ?u-PA ?u-PAR and PAP were measured by ELISA . Gen e transcription of t-PA?u-PA mRNA were detected by Real-time RT-PCR. Results:The plasma levels of u-PA?u-PAR and PAP were elevated in gastric or intestina l cancer patients ,while u-PA was markedly elevated in patients with local infi ltration?lymph node involvement or distal metastasis. u-PA mRNA was higher and t-PA was lower in gastric or intestinal cancer compared to normal tissue. Conclusions:Hyperfibrinolysis was an important factor related with m etastasis potential of gastric or intestinal cancer. t-PA may be a character of well differentiated tissue.更多还原