【摘要】 目的　研究大鼠脑缺血再灌注后脑组织中水孔蛋白 - 4 (AQP- 4 )的表达与脑水肿的关系。方法　采用大鼠大脑中动脉栓塞 (MCAO)模型 ,经免疫组化法测定 AQP- 4表达阳性细胞数 ,干、湿重法测定脑水含量 ,脑水肿以脑水含量评价。结果　脑缺血再灌注后 12 h,脑缺血坏死周边区 AQP- 4表达阳性细胞及脑水含量增加 ,于 4 8～ 12 0 h 达到高峰 ,16 8h后仍处于较高水平 ,两者之间呈正相关 (r=0 .95 8,P<0 .0 1)。结论　脑缺血再灌注后AQP- 4参与脑水肿的发生 ,为脑缺血再灌注损伤的重要因素之一。更多还原

【Abstract】 Objective To study the relationship between the immunohistochemical(IHC) expression of AQP-4 and the brain edema after cerebral ischemic reperfusion in rats. Methods The IHC expressions of AQP-4 in the ischemic regions were determined using immunohistochemistry and the brain water content measure by using the dry-wet weight assay. The level of brain edema was assayed by measuring brain water content. Results The IHC expression of AQP-4 and the brain water content increased significantly 12h after cerebral ischemic reperfusion,peaked at 48～120h and kept the higher levels after 168h. There was a positive correlation between the IHC expressions of AQP-4 and the brain water contents (r=0.958,P<(0.01)). Conclusion The IHC expression of AQP-4 might take part in the formation of the brain edema. The study suggests AQP-4 may play an important role in injury after cerebral ischemic reperfusion.更多还原