【摘要】 目的探讨喉癌组织中Survivin和CD44V6蛋白的表达及其与各临床病理参数的关系。方法应用免疫组织化学技术(SP法)和图像分析技术检测54例喉癌、癌旁正常组织及20例舌腭弓黏膜组织中Survivin和CD44V6蛋白表达水平,并结合临床病理特征进行分析。结果①Survivin在喉癌中66.7%(36/54)阳性表达,癌旁正常组织40.7%(22/54)阳性表达,在20例对照组(正常舌腭弓黏膜组织)中Survivin均为阴性。喉癌组、癌旁组及对照组间的阳性指数(positiveindex,PI)差别有统计学意义(P<0.01)。Survivin的PI与喉癌不同分化程度、临床分期及有、无颈淋巴结转移明显相关(P<0.01或P<0.05),与喉癌的生长位置无明显相关(P>0.05)。②CD44V6在喉癌及癌旁组阳性表达率分别为70.4%(38/54)、24.1%(13/54),对照组阳性表达率为10.0%(2/20),三组间PI的差异有统计学意义(P<0.01)。CD44V6的PI在喉癌的不同分化程度和在有、无颈淋巴结转移间的差异有统计学意义(P<0.01),而与临床分期及喉癌的生长位置无明显相关(P>0.05)。③Survivin与CD44V6的表达呈正相关(r=0.53,P<0.01)。结论①Survivin的表达上调与CD44V6的高表达可能在喉癌的癌变中起协同作用。②Survivin的存在代表着细胞生物学转化的中间环节,Survivin表达阳性提示癌变的可能,因而可能具有早期诊更多还原

【Abstract】 Objective To investigate the relationship between the expression of Survivin and CD44V6 Protein and the clinicopathologic parameters in laryngeal carcinoma. Methods Immunohistochemical technique (S P) and image analysis were used to detect the expression of Survivin and CD44V6 Protein in 54 cases of laryngeal carcinoma , margins of laryngeal carcinoma and 20 cases of normal mucous membrane of palatoglossal pillar. Results ① Expression of survivin was detected in 66.7% (36/54) in laryngeal carcinoma, 40.7% (22/54) in margins of laryngeal carcinoma and negative in normal mucous membrane of palatoglossal pillar. A significant difference was observed for positive index (PI) in three groups (P< 0.01 ). PI of Survivin was related to histological grades、TNM stages and neck lymphoid node metastasis in laryngeal carcinoma (P< 0.01 or P< 0.05 ). PI of Survivin was not related to position of laryngeal carcinoma (P> 0.05 ). ② Expression of CD44V6 was positive in 70.4% (38/54) in laryngeal carcinoma, 24.1% (13/54) in margins of laryngeal carcinoma and 10.0% (2/20) in the control groups. There was a significant difference for PI in the three groups (P< 0.01 ). PI of CD44V6 was related to histological grades and neck lymphoid node metastasis in laryngeal carcinoma. It had no relationship with TNM stages and position of tumour in laryngeal carcinoma (P> 0.05 ). (3) There was a positive correlation between expression of Survivin and CD44V6 protein (r= 0.53 , P< 0.01 ). Conclusions ① The up regulation of Survivin and high rate of CD44V6 might play synergetic roles in the process of carcinogenesis of laryngeal carcinoma. ② The expression of Survivin stands for the middle course in cell biological transformation and is valuable in the early diagnosis. The expression degree of Survivin has an effect on malignant degree and development tendency of carcinoma. It can indicate prognosis of carcinoma of larynx to a certain extent. The treatment of Survivin gene has a good direction of target, and it may be identified as a potential therapeutic target in carcinoma of larynx. ③ Significant difference was observed for PI of CD44V6 in different histological grades, and PI of CD44v6 was related to nect lymphoid node metastasis. It suggests that laryngeal carcinoma with high rate of CD44V6 has stronger invasion. PI of CD44V6 may indicate poor prognosis to a certain extent for patients with laryngeal carcinoma.更多还原