【摘要】 目的观察慢性心肌损伤过程中小鼠细胞免疫功能的改变。方法BALB/C小鼠随机分成4组,病毒(柯萨奇3m,CVB3m)组、毒素(T鄄2)+病毒组、毒素组和对照组。观察各组小鼠脾淋巴细胞亚群及其在刀豆蛋白A(CoA)刺激下增殖及白细胞介素鄄2(IL鄄2)的分泌水平。结果毒素+病毒组小鼠心肌形成慢性损伤,感染病毒7 d时,毒素+病毒组脾淋巴细胞亚群、淋巴细胞增殖反应及IL鄄2的分泌水平均低于病毒组,14 d后虽有不同程度的提高,但低于病毒组最高值。结论T鄄2毒素染毒小鼠感染CVB3m后,心肌出现慢性损伤,这可能是由于淋巴细胞数量、功能和活性异常,细胞免疫功能紊乱,导致发病高峰延迟,阻碍早期清除病毒及杀伤受染细胞的作用有直接关系。更多还原

【Abstract】 Objective To investigate the change of cellular immune functions during chronic myocardial damage in mice.Methods Mice were randomly divided into4groups:viral groupⅠ,toxin+virus groupⅡ,toxin groupⅢand control groupⅣ.The lmyphocyte subset,IL-2content and lymphocyte proliferation were observed.Results The chronic myocardial damage was found in groupⅡ.Seven days after virus infection,the lymphocyte subpopulation,IL-2content and lymphocyte proliferation ability responsive to CoA stimulation were significantly lower than those in groupⅢ.After14days,the level of above-mentioned three indexes increased in different degrees,but still showed inadequate content compared with the highest level in groupⅠ.Conclusions The chronic myocardial damage induced by CVB3m in T-2infected mice might be related to the down-regulation of lymphocyte number,function,activity and abnormal cellular immune function and delay the onset of disease and encumber the early elimination to virus and infected cells.更多还原