【摘要】 目的　检验 5种中草药提取物 (5 ECH)对大鼠的减肥降脂作用及其机制。方法　喂饲高脂饲料制备肥胖高脂大鼠模型 ,5 ECH各设两个剂量为实验组 ,曲美和血脂康为阳性对照组 ,羧甲基纤维素钠为阴性对照组 ,各组均 ig相应药物。 TACHI- 7170 A自动生化分析仪测定血浆甘油三酯 (TG)和总胆固醇 (TC)含量 ;分光光度法和同位素法测定脂肪酸合成酶 (FAS)及脂酰辅酶 A合酶 (ACS)活性。常规称重法测定各组大鼠体重增长指数(BWI)、脂肪质量 (BF)及摄食量 (FI)。结果　 5 ECH对大鼠 BWI、BF、TG、TC和 FI具有剂量依赖性抑制作用 ;并剂量依赖性地抑制 FAS活性、提高 ACS活性。BWI与 FI具有高度相关性 ,TG和 TC水平与 FAS及 ACS具有高度相关性 ,BF与 FI及两种酶的活性具有高度相关性。结论　 5 ECH通过抑制食欲、抑制 FAS活性和促进 ACS活性而对高脂饮食诱导的肥胖大鼠具有显著的剂量依赖性减肥降脂作用。更多还原

【Abstract】 Object To evaluate the effects of the extracts from five kinds of Chinese herbs (5ECH) on weight-reduction and fat-decrease in rats, as well as their mechanisms. Methods Rats were fed by high- calorie food and administrated with two different doses of 5ECH as experimental groups, with Qumei and Xuezhikang as positive control groups, and with carboxymethyl cellulose sodium as negative control group by ig administration with relevant drugs in each groups. Concentrations of triglycerides (TG) and total cholesterol (TC) in the blood were determined by a HITACHI—7170A auto-biochemical analyzer. The activities of fatty acid synthase (FAS) and acyl-coenzyme A synthetase (ACS) were determined by spectrophotometry and isotopic-labeling, respectively. The items of body weight increase (BWI), body fat (BF), and food intake (FI) were determined by regular weighing. Results 5ECH reduces BWI, BF, TG, TC, and FI in a dose-dependent manner inhibits FAS activity, and enhances ACS activity in a dose-dependent manner. The BWI is highly correlated to the FI; the TG and TC levels in the blood are highly correlated to the activities of FAS and ACS, and the BF is highly correlated to both the FI and the two enzymesactivities. Conclusion The 5ECH in this research has significant weight-reduction and fat- decerase effects on rats. The effects are associated with appetite suppression, FAS inhibition, and ACS activation.更多还原