【摘要】 目的 :建立板蓝根颗粒的指纹图谱质量分析方法以保证板蓝根的质量稳定性。方法 :以柱前衍生化HPLC法、BDSC18色谱柱 (4.6× 2 5 0mm)为分析柱、pH6 .4的醋酸钠缓冲液 乙腈 (95∶5 )梯度洗脱、紫外36 5nm下检测建立氨基酸部位的指纹图谱 ,以角余弦系数和距离系数来计算不同批次的样品这间的质量一致程度。结果 :HPLC法的精密度、稳定性和重现性良好 ,图谱中绝大部分色谱峰均达到了基线分离 ;两个系数能够显示样品之间的质量的符合程度。结论 :方法简单 ,准确可靠 ,可控性强 ,可以作为板蓝根颗粒指纹图谱质量控制的一种有效方法。更多还原

【Abstract】 AIM: To establish a fingerprint analytical method for ensuring the quality of Banlangen Granule (Isatis indigotica Fort). METHODS: According to pre column derivatization HPLC method, gradient elution with sodium acetate buffer (pH6.4) acetonitrile (95∶5) in a BDS C 18 column, was applied to detect amino acid at UV 360nm; and the coefficients of cosine of include angle and distance were employed to judge the quality similarity of the samples. RESULTS: The HPLC method is accurate and reproducible and makes almost all the peaks separated; and the two coefficients can show the similarity of each sample. CONCLUSION: The developed method is simple, accurate and operable and can efficiently control the quality of Banlangen Granule.更多还原