【摘要】 目的比较骨髓基质干细胞(BMSCs)与生物衍生骨体内、体外复合所构建的组织工程骨修复山羊胫骨大段骨-骨膜缺损的血管化进程以及血管化与成骨的关系,以探讨修复大段负重骨缺损的最佳途径。方法22只山羊制备成胫骨中段20mm的骨-骨膜缺损,随机分为2组,分别植入BMSCs与生物衍生骨体外构建和体内构建的组织工程骨,常规钢板螺钉内固定,在2、4、6、12周时间点分别用墨汁灌注透明标本、血管面积图像分析和X线片观察血管化过程及成骨情况。结果体外构建组与体内构建组血管化进程无明显区别,各时间点血管面积差异无显著性意义(P>0.05),12周均能达到完全血管化,但X线片见体外构建组新骨形成较快,缺损区密度高,体内构建组以上各变化较前者慢大约2～4周。结论BMSCs与生物衍生骨体外构建和体内构建的组织工程骨均能快速血管化并成骨,但是体内构建方式成骨较体外构建慢。更多还原

【Abstract】 Objective To investigate the vascularization of the tissue engineering bone constructed by marrow stromal cells (MSCs) and bio-derived bone in vivo and in vivo so as to find a good way to repair large bone defects. Methods A 20mm tibia defect was made in each of the 22 goats and fixed with a plate. The goats were randomized into 2 groups. The defects of the 2 groups were filled respectively with the tissue engineered bone constructed by MSCs and bio-derived bone in vivo, and with the tissue engineering bone constructed in vivo. The vascularization for each treatment was assessed by physical, histopathological and X-ray examinations at time intervals of 2, 4, 6, 12 weeks after operation. Results There were an almost equal number of vessels in the 2 groups; and there was no statistically significant difference between the group of the tissue engineered bone constructed in vivo and the group of auto-graft(P >0.05). The implants were vascularized completely at 12 weeks. The new bone formed more quickly in the group of tissue engineered bone constructed in vivo. Conclusions The tissue engineering bone constructed by marrow stromal cells (MSCs) and bio-derived bone in vivo and in vivo can be vascularized quickly and completely. But the new bone forms more slowly in the case of bone construction in vivo than in the case of bone construction in vivo.更多还原