【摘要】 目的探讨肠三叶因子(TFF3)mRNA在慢性阻塞性肺疾病(COPD)大鼠肺组织中的表达。方法采用气管内注入脂多糖加熏香烟的复合刺激法建立大鼠COPD模型,通过半定量病理分析的方法动态观察大鼠肺组织损伤的变化过程;运用RT-PCR半定量检测大鼠肺组织中TFF3mRNA表达量的变化。结果随着造模时间的延长,平均肺内衬间隔(MLI)逐渐增加,平均肺泡数(MAN)逐渐下降;COPD模型7d、14d、21d和28d组大鼠肺组织中TFF3mRNA的表达量逐渐下降,均低于正常对照组;且TFF3mRNA的表达量与MLI呈负相关,与MAN呈正相关。结论在COPD大鼠模型复制过程中,肺组织中TFF3mRNA表达量下降与其病理损伤的程度可能有一定的关系。更多还原

【Abstract】 AIM: To elucidate the change in TFF3 mRNA in the lung tissue in chronic obstructive pulmonary disease (COPD) rats. METHODS: A model of rat COPD was established by passive cigarette smoking and intratracheal instillation of lipopolysaccharide. The expression of TFF3 mRNA in the lung tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Through a semi-quantitative pathological analysis, mean lung internal lining interval (MLI) in COPD model group was significantly increased compared with healthy control groups; mean alveolar number (MAN) in COPD model group was significantly decreased compared with healthy control group. The expression of TFF3 mRNA decreased significantly in the lung tissue in COPD model group compared with healthy control group. CONCLUSION: The level of TFF3 mRNA expression is possibly related with lung tissue lesion in COPD rats. [更多还原