【摘要】 目的 :探讨肌浆网Ca2 + -ATP酶 (SERCA)和受磷蛋白 (PLB)在原发性高血压发病过程中的变化特点及其相互关系。方法 :提取 2、4、6、8、10、12不同周龄雄性自发性高血压大鼠 (SHR)和正常血压大鼠 (WKY)的心室肌、血管平滑肌、肝脏和肾脏组织的总RNA ,共 2 94个样品 ,利用高通量RNA阵列技术 (RNAarray)检测SERCA和PLB基因在不同周龄SHR和WKY中mRNA表达谱改变。结果 :SHR在 6、8、10、12周龄血压出现显著高于同周龄WKY (均P <0 0 1) ,10、12周龄心室肌重量 /体重比出现显著增加 (均P <0 0 1) ,心肌和血管平滑肌SERCA的表达在 4、6、8、10、12周龄出现显著高于同周龄WKY(P <0 0 5或P <0 0 1)。PLB基因表达在两组间无显著差异 (P >0 0 5 )。心肌的SERCA与PLB表达量比值在 6、8、10、12周龄出现显著大于同周龄WKY(P <0 0 5或P <0 0 1) ,而血管平滑肌的SERCA与PLB表达量比值在 4、6、8、10、12周龄出现显著大于同周龄WKY(P <0 0 5或P <0 0 1)。结论 :肌浆网SER CAmRNA表达改变及SERCA与PLB比例失常是高血压发生和发展过程中重要的分子生物学机制更多还原

【Abstract】 AIM: To explore the relationship between the alteration in gene expression of sarcoplasmic reticulum Ca²⁺-ATPase （SERCA） and phospholamban （PLB） in spontaneously hypertensive rats （SHR）. METHODS: 294 samples of total RNA were obtained from the tissue of ventriculum , aortic smooth muscle, liver and kidney in SHR and normotensive rats （WKY）. RNA array was used to determine the mRNA levels of SERCA and PLB. RESULTS: Compared with age-matched WKY rats, the systolic blood pressure increased higher in 6-week-old SHR （P<0.01）. The cardiosomatic ratio was significantly higher in 10-week-old SHR （P<0.01）, in cardiac sarcoplasmic reticulum, the mRNA levels of SERCA were significantly increased from 4 weeks （P<0.05 or P<0.01）. In the aortic sarcoplasmic reticulum, the mRNA levels of SERCA were significantly increased from 4 weeks to 12 weeks （P<0.05 or P<0.01）. There was no significant change in the expression of PLB between the two groups. The ratio of cardiac SERCA and PLB was significantly increased since 6-week-old （P<0.05 or P<0.05）in SHR. The ratio of aortic SERCA and PLB in SHR was significantly increased since 4-week-old （P<0.05 or P<0.01） vs WKY. CONCLUSION: Our results provided the evidence that the abnormalities of intracellular Ca²⁺ hemostasis in SHR represent the progressive nature of essential hypertension. [更多还原