【摘要】 目的 :应用基因芯片技术研究局灶性脑缺血大鼠脑组织与假手术组大鼠脑组织基因表达谱的差异 ,探索局灶性脑缺血的发病机制。方法 :将 4 0 96种大鼠基因PCR产物用CartesianPixsys 75 0 0点样仪按微矩阵排列制成基因芯片 ;按一步法抽提脑局灶性缺血大鼠脑组织与假手术组大鼠脑组织的总RNA ;将等量的脑局灶性缺血大鼠脑组织与假手术组大鼠脑组织RNA分别逆转录合成以Cy5和Cy3标记的cDNA一链做探针 ,混合后与上述基因芯片杂交。用AxonGenepix 4 0 0 0B扫描仪扫描芯片荧光信号图像 ,用GenepixPro4 .0软件进行数字化处理和分析后比较两种组织基因表达谱的差异。结果 :局灶性脑缺血大鼠与假手术组大鼠脑组织基因表达谱分析 ,发现 2 11个差异表达基因 ,其中 12个基因低表达 ,199个基因高表达。结论 :本研究从脑局灶性缺血大鼠脑组织中筛选出大量的差异表达基因 ,说明这些基因可能参与局灶性脑缺血后脑损伤的发生及发展过程 ,从而为局灶性脑缺血的诊治提供新思路更多还原

【Abstract】 AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia. METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.更多还原