【摘要】 目的:研究亚甲基四氢叶酸还原酶(MTHFR)基因多态,叶酸、蛋氨酸摄入量与结直肠癌易感性的关系。方法:应用PCR-RFLP法,检测126例结直肠癌患者和343例正常对照者的MTHFRC 677T和A1298C两个位点基因多态,比较不同基因型与结直肠癌风险的关系,以及两个多态位点与叶酸、蛋氨酸的联合作用。结果: MTHFR C677T和A1298C突变基因在对照组中的频率分别为39.7%和17.1%。MTHFR A1298C突变基因携带者与野生型相比,患结直肠癌的风险显著降低(OR=0.51,95% CI:0.27～0.95);在叶酸个别摄入量组,MTHFR C677T多态使结直肠癌的风险显著降低(OR=0.23,95% CI:0.06～0.93)。结论: MTHFR A1298C位点多态性是直肠癌的保护因素 ,在叶酸摄入充足的条件下,MTHFR C677T多态是结肠癌的保护因素。更多还原

【Abstract】 Objective:To investigate the relationship between folic acid, methionine, methylenetetrahydrofolate reductases (MTHFR) polymorphisms and colorectal cancer susceptibility. Methods:A case-control study of 126 patients and 343 healthy controls was conducted to investigate the role of MTHFR C677T and A1298C polymorphisms in colorectal cancer. Genotypes of C677T and A1298C polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP). Results: The frequencies of MTHFR C677T and A1298C allele was 39.7% and 17.1%, respectively. After adjustment for age and sex, MTHFR A1298C allele showed significant lower risk of rectal cancer compared to wild types (OR= 0.51,95% CI , 0.27-0.95). MHTFR C677T polymorphism was a statistical significant protective factor(OR= 0.23,95% CI , 0.06-0.93)in risk of colon cancer at the group intake more than 115.64 mg folic acid per day. Conclusion:MTHFR A1298C polymorphism may be associated with reduced risk of rectal cancer , and MTHFR C677T polymorphism with enough folic acid intake may be a protective factor of colon cancer.更多还原