【摘要】 以香榧胚为外植体进行体细胞胚的诱导 ,在SH +6 BA 0 1mg/L +2 4 D 0 5mg/L的培养基上愈伤组织诱导率可达到 6 9 8%;愈伤组织经过数次继代后转移至 1 /2SH +6 BA 0 1mg/L +NAA 0 1mg/L培养基上可诱导体细胞胚的形成 ,诱导率可达 70 3%,体细胞胚在合适的培养基上可继续生长和增殖。这是国内外首次通过组织培养成功获得香榧体细胞胚的报道。更多还原

【Abstract】 Calli were induced from embryo of Torreya grandis, and the highest induction frequency(69.8%) was obtained on SH basal medium supplemented with 6-BA 0.1 mg/L+24-D 0.5 mg/L. After subcultured for several times, somatic embryogenesis at high frequency(70.3%) was obtained on 1/2 SH medium supplemented with 6-BA 0.1 mg/L+NAA 0.1 mg/L. Somatic embryos can keep on growing and proliferating on appropriate medium. It is the first reported that somatic embryos were successfully cultured from T. grandis in the world.更多还原