【摘要】 针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB),与含绿色荧光蛋白基因(gfp)和潮霉素磷酸转移酶基因(hot)的pcAMBIA 1300-SmGF、P的根瘤农杆菌LBA4404共培养,培育出了转基因的蝴蝶兰。经绿色荧光蛋白检测和Southern印迹,证实了再生植株中含gfp基因和hot基因。更多还原

【Abstract】 Transgenic orchid (Phalaenopsis cv. White Hikaru) plants were regenerated by inoculating a needle wounded protocorm-like body ( PLB) with Agrobacterium tumefaciens strain LBA4404 harboring pCAMBIA 1300-SmGFP that carries green fluorescent protein gene (gfp) and hygromycin phosphotransferase gene ( hpt) . The expression and presence of the transgenes, gfp and hpt were assessed by detection of green fluorescent protein under microscope and Southern analysis, respectively.更多还原