【摘要】 以银条‘二细一粗’品种为试材 ,研究了蔗糖浓度、激素组合对茎尖培养和快速繁殖的影响及温度、光照、蔗糖浓度等因素对根状茎离体诱导的影响。结果表明 :茎尖培养较理想的培养基为MS +蔗糖 4 % +6 BA 0 5mg·L-1+NAA 0 1mg·L-1,成苗率可达 74 0 %。茎尖增殖较适培养基为MS +6 BA 3 0mg·L-1+NAA 0 5mg·L-1,每个外植体平均可产生 5 6个芽。根状茎诱导以MS +蔗糖 10 % +6 BA 5 0mg·L-1+CCC 5 0 0mg·L-1培养基 ,2 0℃全黑暗培养效果最好更多还原

【Abstract】 The factors which affect tip meristem culture,rapid propagation and the induction of in vitro stolon of manyflower Betony(Stachys floridana Schuttl.ex Benth.)were studied.The results showed that the optimum medium for tip meristem culture were MS+sucrose 4%+6-BA 0.5mg·L -1+NAA 0.1 mg·L -1 with 74.0%plantlet ratio.MS supplemented with 6-BA 3.0 mg·L -1 and NAA 0.5 mg·L -1 was successful for propagation,each explant produced up to 5.6 seedlings.Total darkness,20℃ and MS medium with 6-BA 5.0 mg·L -1+CCC 500 mg·L -1 were better for the induction of in vitro stolon.更多还原