【摘要】 从土壤、植物落花、细菌污染的组织培养三角瓶里的培养基上及被细菌污染的LB琼脂平板上分离筛选出十几株产类胡萝卜素的菌株,其中从污染的LB琼脂平板上分离的一株菌落颜色为黄色的菌株PBH,分类鉴定为库特氏菌属(Kurthia sp.).菌株在以葡萄糖为碳源,添加番茄汁(3.6 mL/L)、芝麻油(1.6 mL/L)、Na:CO3(6g/L)和磷酸缓冲盐的液体培养基中28℃振荡培养5 d,每mL培养液细胞生物量湿重达到65.57 mg,类胡萝卜素产量达到9.896μg,菌体每g湿重细胞胡萝卜素含量为150.9μg.图10参13更多还原

【Abstract】 A bacterium strain PBH that can produce yellow carotenoids was isolated from the contaminated medium of LB agar plate and was identified as Kurthia sp. Its physiological conditions were investigated. When the strain grew in the medium containing glucose(40 g/L) as carbon source with tomato juice(3. 6 mL/L) , gingili oil(1.6 mL/L) , Na2CO3(6 g/L) and phosphate buffer, at 28℃ with illumination for 120 h, its cell biomass, and carotenoid yield and content could reach 65. 57 mg wet cell per mL medium, 9. 896 μg per mL medium and 150. 9 μg per gram wet cell, respectively. Fig 10, Ref 13更多还原