【摘要】 目的 :建立测定胃灵颗粒中甘草酸含量的方法。方法 :采用高效液相色谱法 (HPLC) ,HyersilC1 8柱 (2 5 0mm× 4.6mm ,5 μm) ;流动相 :甲醇 0 .2mol·L 1 醋酸铵溶液 冰醋酸 (66∶3 3∶1) ;检测波长 :2 5 4nm。结果 :甘草酸在 7.44 4～3 7.2 2 0 μg·mL 1 范围内线性关系良好 ,r=0 .9995 ,回收率为 98.5 8%,RSD =0 .65 %。结论 :该方法简便、准确、可控 ,可作为该产品的质量控制方法。更多还原

【Abstract】 Objective:To establish a HPLC method for determination of glycyrrhizic acid in weiling granules. Method:The content of glycyrrhizic acid was determined by HPLC. The Hypersil C 18 was the column; and methanol-0.2 mol·L -1 ammonium acetate-acetic acid (66∶33∶1) was mobile phase. The detective wavelength was 254 nm. Results:The linearity response within the range 7.444-37.320 μg·mL -1 was good (r=0.999 5). The average recovery was 98.58%,RSD=0.65%. Conclusion:The results show that the method is simple and accurate. It can be used to determine the glycyrrhizic acid in the product.更多还原