【摘要】 目的 :对比观察来氟米特 (LEF)和甲氨蝶呤 (MTX)治疗幼年大鼠关节炎的疗效及副作用。方法 :皮内注射Ⅱ型胶原 ,复制胶原关节炎大鼠模型。将大鼠模型随机分为 3组 ,每组 2 0只 :LEF组 ,每只给予LEF 0 .4mg·d 1 ,泼尼松(Pred) 1mg·d 1 ,ig ,疗程 4周 ;MTX组 ,每只给予MTX 0 .1mg·d 1 ,Predlmg·d 1 ,ig ,疗程 4周 ;对照组 ,0 .9%氯化钠溶液 ,ig ,qd ,共 4周。观察指标为关节炎指数评分、足掌体积、运动平衡能力、踝关节的X线改变、滑膜及肝脏病理改变、血清IgG、C 反应蛋白。结果 :治疗第 1周后 ,LEF组与MTX组比较 ,大鼠脚掌肿胀减轻、平衡能力增强 ,两组差异有显著性 (P <0 .0 5 ) ;治疗 4周后 ,LEF与MTX组大鼠脚掌肿胀均完全消失 ;处死动物 ,IgG、C 反应蛋白、肾、踝关节组织病理改变 ,LEF组与MTX组相比 ,差异无显著性 (P >0 .0 5 ) ;与对照组相比较均差异有显著性 (P <0 .0 5 )。肝组织病理检查 ,MTX组部分大鼠肝脏组织切片苏木精 伊红 (HE)染色可见肝细胞玻璃样变性、点状坏死 ,LEF组与MTX组相比 ,差异有显著性 (P <0 .0 5 )。结论 :来氟米特和甲氨蝶呤治疗幼年大鼠关节炎模型均有明显疗效 ,但LEF起效快 ,毒性低。更多还原

【Abstract】 Objective:To establish a model of collagen induced arthritis(CIA) in rats and investigate the effect of leflunomide and methotrexate on CIA in infancy rats. Methods:We reproduced an experimental arthritis model on healthy wistar rats [female, 4 to 5 weeks old, (150±10) g weight] by intradermal injection of Ⅱ type collagen. After that, all rats were divided into three groups randomly and treated differently: In Group LEF, the rats were treated with LEF+Pred for 4 weeks; in Group MTX, the rats were treated with MTX+Pred for 4 weeks; in Group blank control, rats were treated with placebo for 4 weeks. Then we examined arthritis index, ankle joint volume, balance of movement, the X-ray of the rats’ ankle, the histopathology of ankle joint, liver and kidney, the serum IgG and C-reactive protein. Results:The arthritis index, ankle joint volume, balance of movement, the X-ray of ankle joint and the histopathology of ankle joint showed more significant amelioration in Group LEF and Group MTX than the control group, P<0.05. There was only one rat appearing hyaline degeneration in Group LEF and 6 rats appearing hyaline degeneration and spotting necrosis in Group MTX. There was significant difference in the pathologic results of hepatic tissue between these two groups(P<0.05). Conclusion:LEF and MTX both can inhibit the swollen degree of the rats’ ankles and synovium inflammatory responses and improve the motive function, but LEF has less side effects than MTX.更多还原