【摘要】 目的 考察5—HT_2C受体对孵育海马脑片释放分泌型淀粉样前体蛋白（sAPP）的调节。方法 应用5—HT、特异性5-HT_2C受体激动剂M-110及其特异性拮抗L-107孵育海马脑薄片,及Western blot技术和特异性的针对sAPP氨基末端的单克隆抗体22C11检测释放到孵育液中的sAPP。结果 5-HT及特异性5-HT_2C受体激动剂M—110在一定的浓度范围内呈浓度依赖性地显著增加sAPP分泌;而特异性5-HT_2C受体拮抗剂L-107在一定的浓度范围内则浓度依赖性地显著抑制sAPP分泌。结论 5—HT通过激活5—HT_2C受体调节孵育海马脑片分泌型淀粉样前体蛋白的释放。更多还原

【Abstract】 Aim To determine whether serotonin, a major neurotransmitter in brain, can modulate theproduction of secretory β-amyloid protein precursor （sAPP） by activation of serotonin 5-HT_2c receptor. MethodsThe hippocampal slices of rats were incubated with various concentrations of serotonin, M-110, or L-107. sAPPreleased into the incubation medium were assayed by Western blot analysis assay with monoclonal antibody22C11 for 2 h. Results Various concentrations of serotonin (1.0×10^-2-1.0×10³ μmol·L^-1), M-110, aserotonin 5-HT_2c agonist (1.5×10^-6-1.5×10³ μmol·L^-1), showed positive effect on the production of sAPPwhile L-107, a serotonin 5-HT_2c antagonist (1.0×10^-9-1.0×10³μmol·L^-1), showed negative effect on theproduction of sAPP over controls. Conclusion Serotonin modulates production of secretory amyloid β-proteinprecursor through serotonin 5-HT_2c receptor in incubated rat hippocampal slices.更多还原