【摘要】 目的　以体内吸收和分布特点为指导 ,对传统抗肝炎藏药花锚的成分进行研究 ;探索用此方法进行中草药药效成分研究的可行性。方法　利用高效液相色谱二极管阵列检测方法 ,确定能被吸收和分布在大鼠血液、器官和组织中的花锚成分 ,并利用各种色谱分离技术和波谱鉴定其结构。结果　从花锚乙醇提取物中分离鉴定了能被吸收并分布在大鼠血液、器官和组织中的 6个酮苷元类成分。结论　花锚中的酮苷元类成分是能够在大鼠体内检测到的主要被吸收和分布的成分 ,在消化、吸收和代谢过程中它们很快被转化为酮苷元。因此 ,花锚中酮苷类成分的活性主要是通过转化为酮苷元来实现。利用现代灵敏分析检测手段 ,以天然药物成分的吸收分布特点为指导 ,进行天然药物成分的研究是一种可行并且值得进行深入探索的方法。更多还原

【Abstract】 Aim To investigate the chemical constituents of the Tibetan herbal medicine Halenia elliptica by guidance of in vivo absorption and distribution of the constituents. Methods HPLC with a diode array detector (DAD) and HPLC-MS were used for detecting the constituents of extracts of Halenia elliptica and animal samples. Several kinds of column chromatography were used for the isolation and purification of the main in vivo absorbed and distributed compounds from the extract of Halenia elliptica . Results Six main components detected in the extracts of the animal samples were isolated from the ethanolic extract of Halenia elliptica . Conclusion After rats were treated with different extracts of Halenia elliptica , low polar components xanthone aglycon of Halenia elliptica were clearly observed in the extracts of liver, lipid, blood, hidney, heart and brain tissue of rats, while the polar components xanthone glycosides were detected in very small amounts in the animal samples. The xanthone glycosides can be decomposed into xanthone aglycons during the digestion, absorption and metabolism procedure. Therefore, the in vivo activity of the xanthone glycosides might be exhibited by their decomposed products. It is an accessibly valuable method to investigate chemical components of herbal medicines under the guidance by detecting in vivo absorption and distribution of chemical components of the herbal medicine extract.更多还原