【摘要】 目的　探讨皮质醇及肿瘤坏死因子 -α对脓毒症大鼠的免疫功能的影响。方法　将 40只大鼠随机分为 2组 ,正常对照组、盲肠结扎并穿孔组各 2 0只。外周血行中性粒细胞吞噬功能测定及白细胞移行抑制实验 ,直接放射免疫分析方法及酶联免疫吸附法分别测定血清皮质醇及肿瘤坏死因子 -α。结果　盲肠结扎并穿孔术后 3 6h大鼠出现低皮质醇血症及过多的肿瘤坏死因子 -α,细胞免疫功能减弱 ,胸腺萎缩。结论　低皮质醇血症及过多的肿瘤坏死因子 -α在造成脓毒症大鼠的免疫功能紊乱起重要作用。更多还原

【Abstract】 Objective To study the effects of tumor necrosis factor-α and cortisol on the immune of sepsis rats induced by cecal ligation and puncture.Methods Rats were divided into 2 groups,the normol control group and the CLP model group;Phagocytosis of PMN,thymus index and MII were detected,The serum level of tumor necrosis factor-αand cortisol were detected by means of ELISA and RIA method.Results The level of TNF-α increased after CLP;but level of cortisol was lower than that of control group’after CLP . Phagocytosis of PMN weaked、thymus index decreased and MII increased after CLP .Conclusions CLP may result in immunologic dissonance.the mechanism may be involved in lower cortisol and overwhelming TNF-α.更多还原