【摘要】 目的 :探讨阿托伐他汀对糖尿病大鼠肾脏的保护作用及其机制。方法 :将SD大鼠随机分为正常对照组(C组 )、糖尿病组 (D组 )、阿托伐他汀治疗组 (DT组 )。检测各组大鼠在灌胃给药第 4、第 8周时血糖、糖化血红蛋白(HbA1c)、血胆固醇、三酰甘油、高密度脂蛋白、血肌酐、尿素氮、尿白蛋白、血转化生长因子 (TGF β1)浓度及体重等指标的变化 ,免疫组化检测纤维连接蛋白 (FN)和层黏连蛋白 (LN)表达水平。结果 :第 8周时 ,DT组大鼠血胆固醇、三酰甘油、肌酐、TGF - β1浓度及尿白蛋白排泄率较D组明显减少 (P <0 0 1或 <0 0 5 ) ;免疫组化显示 ,DL组大鼠肾小球FN和LN的表达明显低于D组 (P <0 0 1或 <0 0 5 )。结论 :阿托伐他汀对于糖尿病大鼠肾脏具有部分保护作用 ,其可抑制血TGF - β1浓度的升高 ,下调糖尿病大鼠TGF β1的表达 ,减少细胞外基质的沉积。更多还原

【Abstract】 Objective:To investigate the renal protective effect of atorvastatin in diabetic rats and its mechanism. Methods:30 SD rats were divided randomly into 3 groups:normal control (C) group, STZ-induced diabetic (D) group and atorvastatin-treated (DT) group. Plasma glucose, HbA1c, serum cholesterol, triglyceride, high density lipoprotein, serum creatinine, blood uria nitrogen, urinary albumin, and serum TGF-β1 were observed respectively at the fourth and eighth week of treatment, and the expressions of fibronectin and laminine in the kidney were measured by immunohistochemistry. Results:At the eighth week, serum cholesterol, triglyceride, creatinine and TGF-β1 level, and urinary albumin excretion in DT group were significantly lower than those in D group(P<0.01 or <0.05). Immunohistochemistry results showed that the expressions of fibronectin and laminine in glomerulus and laminine in DT group were much lower than those in D group (P<0.01 or <0.05). Conclusion:Atorvastatin has some renal protective effects on diabetic rats. This might result from decreasing serum TGF-β1 level, down-regulating TGF-β1 expression and reducing the deposition of glomerular extracellular matrix.更多还原