【摘要】 目的 :研究吗啡对培养的大鼠胶质细胞瘤C6的增殖抑制作用及其机制。方法 :3H -TdR掺入法测定吗啡对细胞增殖的影响 ;RT -PCR方法观察吗啡对细胞PCNA基因表达的影响。免疫组织化学法观察吗啡对细胞PCNA蛋白质水平的影响。结果 :不同浓度吗啡 (0 0 0 1- 10 0mg·L- 1 )作用于培养C6细胞 2 4h后 ,3H -TdR掺入量剂量依赖性降低。不同浓度吗啡 (0 0 0 1- 10 0mg·L- 1 )作用于培养C6细胞 2 4h后 ,细胞PCNAmRNA的相对含量与对照组相比均明显减少 (P <0 0 1)。 10mg·L- 1 吗啡作用细胞 6 - 4 8h后 ,不同时间点吗啡组细胞的PCNAmRNA的相对含量与对照组相比均明显减少 (P <0 0 1)。10mg·L- 1 吗啡作用细胞 2 4h后 ,与相邻吗啡组比较细胞内PCNA蛋白质水平明显降低 (P <0 0 1)。结论 :吗啡对胶质细胞瘤C6的增殖具有抑制作用 ,其机理可能与吗啡使细胞中PCNA的基因表达水平降低有关。更多还原

【Abstract】 Objective: To study the effect and mechanism of morphine on proliferation of rat C6 glioma cells. Methods: 3H-TdR incorporation was used to determine the effect of morphine on cell proliferation. The effect of morphine on PCNA gene expression in C6 was determined by RT-PCR and immunohistochemistry. Results: Morphine at dose 0 001-100 mg·L -1 decreased 3H-TdR incorporation in a dose-dependent manner. The level of PCNA gene expression in C6 glioma cells decreased after exposure to morphine 0 001-100 mg·L -1 for 24 hours and also decreased at all time points after exposure to morphine 10 mg·L -1 for 6-48 hours. The protein level of PCNA in C6 glioma cells decreased after exposure to morphine 10 mg·L -1 for 24 hours. Conclusion: Morphine has an inhibitory effect on proliferation of rat C6 glioma cells and the mechanism may be related to its inhibitory effect on gene expression of PCNA.更多还原