【摘要】 为检测注射用重组双功能水蛭素半成品中的DNA残留量 ,应用地高辛标记重组双功能水蛭素工程菌DNA ,并以此为DNA探针进行点杂交 ,同时做特异性及灵敏度实验。结果证明 ,该方法检测最低限值可达1pg ,且重复性好 ,特异性强 ,操作较简便 ,可用于双功能水蛭素制备工艺的质控及半成品的检定更多还原

【Abstract】 DNA of engineering bacteria of recombinant double functions hirudin were labeled by Digoxin and used as probe for dot hybridization to detect residual DNA in semi-finished products of recombinant double functions hirudin for injection. At the same time, specificity and sensitivity experiments were performed. It was proved by experimental results that the lowest volume of detection was up to 1 pg. This method had good repeatability and specificity, simple operation, and was competent for quality control in preparation technique of recombinant double functions hirudin and assay for semi-finished products.更多还原