【摘要】 采用自溶、35 %硫酸铵分部沉淀、CM 纤维素柱层析、羟基磷灰石柱层析由动物肝脏中分离纯化β D 葡 萄糖醛酸苷酶 ,获得比活 2 6 70 0U/mg的纯酶 ,活力回收率为 4 2 .8% ,纯化的酶经SDS PAGE呈单一条带。该酶以 β D 酚酞葡萄糖醛酸苷为底物的Km值为 2 .0× 10 -4mol/L ,以对硝基苯酚葡萄糖醛酸苷为底物的Km值为 2 .5× 10 -4mol/L。在 pH值 5～ 6的范围内 ,该酶的活力较高 ,最适 pH值为 5 .5 ,在pH值 5 .5时稳定性最好。在 30～ 5 0℃范围内酶活力均较稳定更多还原

【Abstract】 Using means of autolysis, 35（）% saturated ammonium sulfate fractionation, CM-cellulose column chromatography, and Hydroxyapatite chromatography to purify β-D-Glucuronidase from bovine liver, an 42（）% yield of enzyme with a specific activity of approximately 26（）700（）U/mg was obtained. The purified enzyme shows single band on SDS-PAGE. The optimum pH is 5.5, and the enzyme was most stable from pH5.0 to pH6.0. The K_m with phenolphthalein glucuronide and nitrophenyl glucuronide as substrates were 0.2（）mmol/L and 0.25（）mmol/L respectively.更多还原