【摘要】 目的:采用毛细管电泳法同时测定发酵液中的苯乙酮酸和扁桃酸对映异构体的含量。方法:运行缓冲液为100 mmol·L-1 Tris-磷酸缓冲液(pH 7.6,含150 g·L-1羟丙基-β-环糊精);运行电压20 kV;柱温20℃;检测波长214 nm。结果:扁桃酸样品(0.75 mmol·L-1)的分离度(Rs)可达1.29;线性范围为0.065-1.0 mmol·L-1(r=0.998),最低检测限为0.01mmol·L-1(S/N=3);实际样品的平均回收率为99.2%;2个对映体迁移时间的RSD均小于1.0%,峰面积的RSD均小于3.0%。结论:采用该方法分析生物不对称合成的手性扁桃酸发酵样品,具有准确、经济、快速、简便等特点。更多还原

【Abstract】 Objective: To develop a method for the determination of phenylglyoxylic acid and mandelic acid enanti-omers in ferments by capillary electrophoresis. Methods; The buffer solution used in this method was 100 mmol · L -1. Tris - phosphate ( containing 150 g · L -1 hydroxypropyl - β - cyclodextrin, pH 7.6). The running voltage was 20 kV. The column temperature was 20 ℃ and the detection wavelength;214 nm. Results;The resolution degree ( Rs) of R - and S - mandelic acid (0. 75 mmol · L -1 ) was 1. 29. A linear calibration was obtained in the range of 0. 065 -1.0 mmol · L -1 of R- and S - mandelic acid( r = 0. 998 ) and the average recovery was 99. 2%. The minimum detectable concentration of R, S - mandelic acid was 0. 01 mmol · L -1 ( S/N = 3 ). The relative standard deviation (RSD) value for peak areas of R -and S -mandelic acid were 2. 7% and 2. 4% Respectively. Conclusion: The quantitation of chiral mandelic acid samples in the ferments from biosynthesis by Sacharomyces Cerevisiae was tried, and as shown by the results, the method is precise, simple and cost - effective.更多还原