【摘要】 以蚊净香草嫩叶和叶柄为外植体进行培养,筛选合适的外植体与诱导、增殖和生根的最佳培养基。结果表明,叶片外植体在MS + 6-BA 2.0mg/L + NAA 0.5mg/L培养基最适于诱导愈伤组织和不定芽;MS + 6-BA 1.0mg/L + NAA 0.3mg/L培养基有利于不定芽增殖;无根苗在含有低浓度无机盐和生长素水平的生根培养基上易生根,生根率高达100%。更多还原

【Abstract】 Taking the petioles and leaves of Mozzie buster as explants, tissue culture and rapid propagation were studied. The results showed that the leaves cultured on MS media supplemented with 6-BA 2.0mg/L and NAA 0.5mg/L was best for inducing callus and adventitious buds, and MS media with 6-BA 1.0mg/L and NAA 0.3mg/L was suitable for subculture of adventitious buds. The microshoots rooted in low salt media containing low level of auxin, rooting rate up to 100%.更多还原