【摘要】 目的 :观察从生物工程技术得到的灵菌红素对人胰腺癌 8898细胞增殖抑制的药效学作用 ,并探讨其作用的部分机理。方法 :用MTT法测定 8898细胞的存活率和抑制率 ,用流式细胞仪分析细胞的周期变化和凋亡细胞的百分比 ,用HPLC检测人胰腺癌 8898细胞内灵菌红素的浓度 ,用琼脂糖凝胶电泳对DNA段片化进行分析。结果 :灵菌红素5mg·L-1的培养液中 8898细胞出现凋亡早期的形态学特征 ,半数抑制浓度 (IC50 )为 30mg·L-1。而3T3细胞却未显示出该作用。研究表明灵菌红素抑制细胞的增殖 ,与抑制S期细胞的DNA复制及调控细胞的增殖周期相关。同时 ,细胞凋亡的产生与实验药物呈正相关。HPLC结果显示 ,灵菌红素的药理学作用与细胞内药物浓度相关。结论 :灵菌红素能有效的进入细胞内 ,抑制细胞的增殖 ,其机理与诱导细胞凋亡和调控细胞的增殖周期相关。更多还原

【Abstract】 AIM: To investigate the inhibitive effects of prodigiosin on the multiplication of human malignant pancreas cancer cell 8898,and its mechanism. METHODS: MTT assay was used to measure the rate of viability and inhibition of 8898 cells;the change of cell cycle and the percent of apoptosis were analyzed by FCM;the concentration of prodigiosin in 8898 cells was measured HPLC;and the fragmented DNA was analyzed by agarose gel electrophoresis. RESULTS: In the culture medium containing 5 mg·L -1 prodigiosin,8898 cells appeared the morphological character of early stage of apoptosis. Half inhibition concentration (IC_ 50 ) was 30 mg·L -1 . But to 3T3 cells the drug had no obvious effect. The inhibitive effect of prodigiosin on multiplication of cancer cells was related to the inhibiting of DNA replication in S stage and regulating of cell cycle. The results of HPLC showed that pharmacological effect of prodigiosin dependent on drug concentration in cells. CONCLUSION: Prodigiosin can enter cells and inhibit the multiplication of cells. The mechanism may be related to inducing apoptosis and regulating cell cycle.更多还原