【摘要】 综述检测CYP2A6基因多态性的PCR技术的基因型方法。CYP2A6基因多态性存在于白种人、东方人及非洲裔美国人群中 ,包括CYP2A6 1至CYP2A6 12 ,总共 12个变异等位基因。本文列举了检测CYP2A6基因型的不同的DNA来源、引物和限制性内切酶。已开发PCR技术结合诊断性限制性内切酶分析 (RFLP)如两步PCR和一步PCR RFLP方法检测CYP2A6基因型。此外 ,尚有PCR SSCP +RFLP及直接DNA测序方法。更多还原

【Abstract】 To review PCR based genotyping methods for genetic polymorphism of CYP2A6 gene. Genetic polymorphism of CYP2A6 has been found in Caucasians, Oriental and African American population, which includes from CYP2A6*1 to CYP2A6*12, totally 12 variant alleles. In this article, different DNA source, primers and restriction enzymes for CYP2A6 genotypes were reviewed. Protocols for identification of CYP2A6 genotypes with PCR based techniques combined with diagnostic restriction enzyme digestion such as two step and one step PCR RFLP methods have been developed. Besides, methods of PCR SSCP combined with RFLP and direct DNA sequencing are also introduced.更多还原