【摘要】 目的　探讨不同乙醇脱氢酶 3(ADH3)基因型对乙醇体内氧化的影响。方法　采用PCR扩增反应测定ADH3基因型 ,将 2 2名健康受试者分为ADH31 -1 ,ADH31 -2 和ADH32 -2 3组。受试者一次po 0 .3g·kg-1 乙醇后 ,采用高效液相色谱荧光法测定 4h内唾液和全血中的乙醛浓度经时过程。结果ADH31 -1 组的乙醛唾液cmax为 (1 2 .5± 2 .3) μmol·L-1 ,AUC为 (1 .4± 0 .4)mmol·min·L-1 ;ADH31 -2 组唾液cmax为 (9.4±1 .7) μmol·L-1 ,AUC为 (1 .1±0 .3)mmol·min·L-1 ;ADH32 -2 组唾液cmax为 (8.7± 2 .2 )μmol·L-1 ,AUC为 (0 .93± 0 .1 9)mmol·min·L-1 。ADH31 -1 组的唾液中乙醛浓度显著高于ADH31 -2 和ADH32 -2 组 ,但全血中的组间差异不如唾液中明显。结论　基因型为ADH31 -1 的个体在摄入乙醇后唾液中乙醛量显著增加 ,提示ADH31 -1 个体酗酒时由乙醛引发病理损害的风险性较大更多还原

【Abstract】 AIM To explore the effect of different genotype of alcohol dehydrogenase 3(ADH3) on the oxidation of ethanol in vivo. METHODS Twenty two volunteers were divided into ADH3 1-1 , ADH3 1-2 , ADH3 2-2 groups using PCR amplification. Volunteers were given an oral acute dose of 0.3 g ethanol·kg -1 . Salivary and blood acetaldehyde concentrations were measured using HPLC with fluorescence detection over a period of 4 h. RESULTS In ADH3 1-1 group salivary c max and AUC were (12.5±2.3) μmol·L -1 , (1.4±0.4) min·mmol·L -1 ; In ADH3 1-2 group salivary c max and AUC were (9.4±1.7)μmol·L -1 , (1.1± 0.3)min ·mmol·L -1 ;In ADH3 2-2 group salivary c max and AUC were (8.7±2.2) μmol·L -1 , (0.93 ±0.19) min·mmol·L -1 . There was not so significant difference in acetaldehyde content in blood as in salivary among the three groups.CONCLUSION Salivary acetaldehyde concentrations are significantly increased in individuals with ADH3 1-1 , it may implicate that the risk of pathological impairment induced by acetaldehyde is enhanced in individuals with this genotype.更多还原