【摘要】 目的　探讨Ki -67和BRCA1在散发性乳腺癌中的表达 ,评价其在乳腺癌诊断、转移潜能及预后判断中的作用。方法　应用S -P免疫组化法检测 3 0例正常乳腺组织、3 0例异型增生的乳腺癌旁组织和 5 0例乳腺癌组织Ki -67和BRCA1的表达情况。结果　乳腺癌组织Ki-67阳性表达率为 92 % (4 6/ 5 0 ) ,显著高于异型增生及正常乳腺组织 (P均 <0 .0 1)。正常乳腺组织BRCA110 0 % (3 0 / 3 0 )呈阳性表达 ,强阳性表达率为 70 % (2 1/ 3 0 ) ,异型增生BRCA1阳性表达率为 80 % (2 4/ 3 0 ) ,强阳性表达率为 3 6.67% (11/ 3 0 ) ,定位于细胞核。而乳腺癌BRCA1阳性表达率为 5 8% (2 9/ 5 0 ) ,无一例强阳性表达 ,其亚细胞定位除细胞核外 ,也见于胞浆、胞核胞浆。Ki -67强阳性表达者术后 1、3、5年生存率显著低于弱阳性或阴性表达者 (P <0 .0 5 ) ;BRCA1表达正常的乳腺癌病人术后 3、5年生存率显著高于减弱者 (P <0 .0 5 )。结论　检测Ki-67和BRCA1表达有助于乳腺癌的诊断和转移潜能及预后的判断。更多还原

【Abstract】 Objective To investigate the expression of Ki-67 and BRCA1 in sporadic breast cancer and the roles that they played in diagnosis of breast cancer, metastasis potentiality and in prediction of disease prognosis.Methods An S-P immunohistochemical method was used to detect the expression of Ki-67 and BRCA1 in 30 cases of normal breast tissues, 30 allotype proliferation tissues adjacent to breast cancer and 50 breast cancer tissues. Results The expression of Ki-67 were positive, 92% (46/50) in the breast cancer tissues, which was markedly higher than those in the allotype proliferation tissues and the normal breast tissues, all the p values were <0.01. 100% (30/30) of BRCA1 expressed in normal breast tissues were positive and the strong positive expression rate was 70% (21/30); and in allotype proliferation tissues, the positive expression rate of BRCA1 was 80% (24/30) and the strong positive rate was 36.67% (11/30), locating in the cell nucleus. 58% (29/50) of BRCA1 in breast cancer tissues were positive, no strong positive expression, the subcellulars located in the cell nucleus as well as in cytoplasms,cell nucleus cytoplasm. In comparison of the 1, 3 and 5-year survival rates between patients with strong positive expression of Ki-67 and patients with weak positive or negative expression of Ki-67, the differences were statistically significant (P<0.05); and in comparison of the 3 and 5-year survival rates after surgery between breast cancer patients with normal BRCA1 expression and patients with weak expression, the differences were also statistically significant (P<0.05). Conclusion The Ki-67 and BRCA1 indexes can help to diagnose breast cancer and judge the metastasis potentiality and predict the disease prognosis.更多还原