【摘要】 目的　探讨动脉内膜损伤后Ⅰ、Ⅲ型胶原、金属蛋白酶 2 (MMP 2 )、金属蛋白酶 9(MMP 9)的动态变化及与细胞迁移、内膜增厚的关系。方法　在兔腹主动脉下端内膜损伤后狭窄的模型上 ,采用免疫组化、原位杂交的方法检测Ⅰ、Ⅲ型胶原、MMP 2、MMP 9的表达 ,并对结果进行图像分析。结果　内膜损伤后 1周Ⅰ型胶原的表达增加 ,4个月达到最大值。Ⅲ型胶原mRNA在正常血管的表达极低 ,损伤后 1周迅速增加 ,至 4周达到峰值 ,以后逐渐下降。MMP 2、MMP 9在损伤后 1周即表达 ,4周达到峰值 ,以后逐渐降低。结论　Ⅰ、Ⅲ型原的交替分泌可能是导致内膜伤后狭窄的重要原因。MMP 2、MMP 9损伤早期表达可能与细胞向内膜迁移有关 ,晚期表达减少导致胶原积聚更多还原

【Abstract】 Objective To investigate the expression of type Ⅰ,type Ⅲ collagen,MMP-2,MMP-9 in the repaired arteries after injury and their roles in restenosis.Methods Stenosis models of ventral aorta after arterial intimal injury were established in rabbits.Immunohistochemistry and in situ hybridization were used to evaluate the expression of type Ⅰ,type Ⅲ collagen,MMP-2 and MMP-9 in injured intima.Results The obvious expression of type Ⅰ collagen started about 7 to 14 days after intimal injury,and remained increasing in the following 4 months.The expression level of type Ⅲ collagen,MMP-2 and MMP-9 were very low in normal aorta but increased rapidly at 7th day after intimal injury and reached the maximum at 28th day.Conclusion Overexpressions of type Ⅰ,type Ⅲ collagen and the proportion between these two kinds of collagen and low-expression of MMP-2,MMP-9 are considered to play an important role in the development of restenosis.更多还原