【摘要】 目的　观察合肥地区 2型糖尿病患者G蛋白 β3 亚单位C82 5T (GNβ3 C82 5T)多态性 ,探讨其与 2型糖尿病(T2DM)的关系。方法　采用聚合酶链式反应和限制性片段长度多态技术 (PCR RFLP)来检测单纯T2DM患者 191例 ,T2DM合并高血压患者 (HT) 10 2例和对照人群 70例的GNβ3 C82 5T基因型 ,并进行病例对照统计分析。结果　对照组基因型频率CC、CT、TT为 34 3%、5 0 %、15 7% ,等位基因频率C、T分别为 5 9%、4 1% ;T2DM组基因型频率CC、CT、TT为 32 5 %、4 9 7%、17 8% ,等位基因频率C、T分别为 5 7%、4 3% ;T2DM +HT组CC、CT、TT为 2 6 5 %、5 0 %、2 3 5 % ,等位基因频率C、T分别为 5 1%、4 9% ;三组间基因型频率比较 χ2 =2 6 88,P =0 6 11;三组间等位基因频率比较 χ2 =2 5 86 ,P =0 2 74 ,C82 5T多态性与对照组比较差异无显著性。结论　在本人群中GNβ3 C82 5T多态性与T2DM无关系。更多还原

【Abstract】 Objective To explore the association between C825Tpolymorphism of a human G-protein β 3 subunit (GNβ 3)and type2 diabetes mellitus(T2DM). Methods The genotype of GNβ 3 C825T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique in the Chinese Han population of Hefei region including 70 normal subjects(con),191 T2DM with Hypertension(T2DM+HT). We compared the genotype and allele frequencies in these groups and analyzed their relationships by case-control method. Results There were no significant difference in the frequencies of genotype and allele among these groups of con, T2DM, T2DM+HT(con:CC/CT/TT=34.3%/50%/15.7%;T2DM:CC/CT/TT=32.5%/49.7%/17.8%;T2DM+HT:CC/CT/TT=26.5%/50%/23.5%,the genotype frequency χ2=2.688,P=0.611;the allele frequency χ2=2.586,P=0.274). Conclusion The GNβ 3C825T polymorphism has no relationship to T2DM for the population.更多还原