Objective To evaluate the effect of DMSO on the isolation of human cytomegalovirus. Methods Eleven urine specimens from clinical kidney transplant recipients and children of cerebral palsy, were inoculated into two groups of human fibroblast cells for HCMV isolation. The growth medium of one group contained a final concentration of 1% DMSO; another group was used as control. PCR was used to evaluate the efficacy of isolation. Results Among 11 samples, CPE appeared in two groups, and the samples was unani...
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