【摘要】 对国内外斑马鱼基因工程研究进展 ,包括最近获得的大批转基因斑马鱼品系、靶向筛选的转基因斑马鱼、斑马鱼转基因技术的发展和斑马鱼基因工程的热点问题与应用前景进行了综述。指出我国已建立日趋成熟的斑马鱼转基因技术、细胞核移植技术和染色体组操作技术 ,具有斑马鱼细胞遗传学和胚胎学基础 ,我国有望在不久的将来获得定向整合的基因工程斑马鱼 ,并推动生物技术应用研究领域的进步和发展更多还原

【Abstract】 The research progress of zebrafish gene engineering is reviewed,in including the recent explosion status of transgenic zebrafish lines,transgeneic zebrafish in targeted screens,the development of transgenic technology in zebrafish as well as the current hotspots and future perspective of zebrafish gene engineering.By now the zebrafish gene engineering,nuclear transplantation and chromosome set manipulation technologies have been established in China. With firm foundation of zebrafish cytogenetics and embryology,we will obtain gene engineering zebrafish with gene targeting integration and promote the advancement and development of biotechnology in applied research field in near future.更多还原