【摘要】 对被认为是自然杂交种的绿绒蒿植物Meconopsis×cookeiG .Taylor及其可能的亲本红花绿绒蒿(M punicea)和五脉绿绒蒿 (M .quintuplinervia)的核糖体DNAITS区进行了序列测定 ,所得序列的长度为 6 6 7～ 6 6 8bp ,其中红花绿绒蒿的序列长度为 6 6 7bp ,另外两个种的序列长度均为 6 6 8bp。利用软件ClustalX对所得序列进行排序和碱基比较 ,排序后的序列长度为 6 6 8bp ,其中ITS1长度为 2 5 4bp ,5 8S长度为 16 2bp ,ITS2长度为 2 5 2bp。整个ITS区序列共有 16个变异位点 ,占序列总长度的 2 4 0 % ,其中ITS1的变异位点 9个 ,占 5 6 2 5 % ,占整个序列长度的 1 35 % ;ITS2的变异位点 6个 ,占 37 5 0 % ,占整个序列长度的 0 89% ;5 8S的变异位点 1个 ,占 6 2 5 % ,占整个序列长度的 0 15 %。分析结果表明 ,M .×cookei同时具有红花绿绒蒿和五脉绿绒蒿的两种ITS序列 ,也就是说M .×cookei与红花绿绒蒿和五脉绿绒蒿之间在ITS基因上的变化规律符合孟德尔遗传学定律 ,从而从分子水平上证明M .×cookei是红花绿绒蒿和五脉绿绒蒿的杂交后代。更多还原

【Abstract】 The Nuclear Ribosomal DNA internal transcribed spacers (ITS) region sequences from a putative natural hybrid species Meconopsis×cookei and its possible parents M.punicea and M.quintuplinervia were obtained by using direct sequencing method.The sequence length of ITS region (including ITS1,5.8S and ITS2) is 667 bp for M.punicea ,668 bp for M.×cookei ,and 668 bp for M.quintuplinervia. The sequences were aligned by the software Clustal X,and the bases per locus were compared by using software with manual method.The aligned sequence length is 688 bp,of which ITS1 is 254 bp,5.8S is 162 bp,and ITS2 is 252 bp.16 variable loci were detected from the aligned sequence,approximately 2.40% to the whole sequence length,of which ITS1 has nine variable loci (56.25%),ITS2 has six variable loci (37.50%),and 5.8S has one variable locus (6.25%).The results show that M.×cookei have two kinds of ITS sequences contributed from two species M.puniceaa and M.quintuplinervia ,i.e.the variation of ITS gene among M.×cookei , M.puniceaa and M.quintuplinervia is congruence with the Mendel’s genetics law.Therefore,the molecular evidences indicate that M.×cookei is a hybrid origin from M.puniceaa and M.quintuplinervia .更多还原