【摘要】 利用抑制差减杂交 (SuppressionSubtractiveHybridization ,SSH)和高密度点阵膜技术研究小麦 2叶幼苗期水分胁迫诱导表达基因。通过筛选具有 15 30个克隆的SSH文库 ,获得 181个阳性克隆。序列同源性比较和功能查询结果发现 ,83 2 %的水分胁迫诱导表达基因分别与不同逆境胁迫条件下表达的基因具有较高的同源性 ,这些基因在生物体内的功能都是直接或间接对细胞遭受逆境胁迫起保护作用。其中 17个EST未找到同源性较高的匹配序列 ,已经在GenBank注册。用反向Northern、RT PCR和Northern进一步检验所获得的功能已知EST ,初步建立了小麦幼苗期水分胁迫诱导的基因表达谱更多还原

【Abstract】 In the present research,suppression subtractive hybridization (SSH) and high density membrane techniques were employed to analysis genes induced by water stress in wheat seedling at 2-leaf stage.The purpose was to comprehensively understand the genetic bases of drought resistance and to find the key genes related to drought resistance in wheat.A total of 181 positive clones were obtained by screening the SSH library including 1 530 individual recombinant clones.The result of the sequence homologous comparison and function querying showed that 83.2% different ially expressed genes were high homologous with those induced by abiotic or biotic stresses in plant and their functions were to protect the cell directly or indirectly from stress strain.Seventeen differentially expressed ESTs found first were registered in GenBank.After further identifying by reverse Northern,RT-PCR and Northern,the gene expression profile induced by water stress in wheat seedling was preliminarily revealed.更多还原