【摘要】 目的　研究L 型钙通道阻断剂对曲马多镇痛作用的影响。方法　选用小鼠热板实验和醋酸扭体实验作为评价方法。腹腔注射曲马多观察该药在不同模型中的镇痛作用。维拉帕米 ,尼莫地平或硝苯地平分别与阈下剂量曲马多合用 ,观察这 3种L 型钙通道阻断剂对曲马多镇痛作用的影响。结果　热板实验中 ,曲马多 (10 ,2 0 ,4 0mg·kg-1)剂量依赖性地延长小鼠舔后足或跳跃的潜伏期 ;维拉帕米可增强曲马多的镇痛作用。醋酸扭体实验中 ,曲马多 (2 ,5 ,10mg·kg-1)显著减少小鼠扭体反应的次数 ;维拉帕米、尼莫地平和硝苯地平均可剂量依赖性地增强曲马多的镇痛作用。结论　L 型钙通道阻断剂维拉帕米、尼莫地平和硝苯地平对曲马多的镇痛作用有一定的增强作用。L 型钙通道介导的胞外钙内流可能参与了曲马多的镇痛机制更多还原

【Abstract】 Aim To investigate the effects of L-type calcium channel blockers on tramadol-induced analgesia.Methods Hot-plate test and writhing test were used to measure the analgesia induced by tramadol. Verapamil, nimodipine or nifedipine was co-administrated with tramadol to determine its effects on tramadol analgesia.Results Tramadol (10, 20, 40 mg·kg -1 in hot-plate test or 2, 5,10 mg·kg -1 in writhing test) produced significantly analgesia in hot-plate test and writhing test. Co-administration of verapamil and tramadol prolonged the latency of pain response of mice in hot-plate test.In writhing test, verapamil, nimodipine and nifedipine could potentiate the analgesic effect of tramadol in a dose-dependent manner.Conclusion L-type calcium channel blockers can potentiate tramadol-induced analgesia. Calcium influx mediated by L-type calcium channels may be involved in tramadol-induced analgesia.更多还原