【摘要】 目的　研究腺病毒 (adenovirus,Ad)介导mIFN β基因转染的B16小鼠黑色素瘤细胞的体内致瘤性及抗肿瘤作用。方法　用携带mIFN β基因的重组腺病毒载体体外转染B16细胞 ,将该细胞接种于小鼠 ,观察其在小鼠体内的致瘤性及对野生型B16细胞的致瘤性有无影响。结果　经腺病毒介导 ,mIFN β基因成功地导入B16细胞并获得有效表达 ;转mIFN β基因的B16细胞的体内致瘤性明显降低 ,并能抑制对侧野生型B16细胞的致瘤性。结论　该研究结果提示利用腺病毒载体携带IFN β基因来开发瘤苗治疗肿瘤具有良好的应用前景。目的　研究腺病毒 (adenovirus,Ad)介导mIFN β基因转染的B16小鼠黑色素瘤细胞的体内致瘤性及抗肿瘤作用。方法　用携带mIFN β基因的重组腺病毒载体体外转染B16细胞 ,将该细胞接种于小鼠 ,观察其在小鼠体内的致瘤性及对野生型B16细胞的致瘤性有无影响。结果　经腺病毒介导 ,mIFN β基因成功地导入B16细胞并获得有效表达 ;转mIFN β基因的B16细胞的体内致瘤性明显降低 ,并能抑制对侧野生型B16细胞的致瘤性。结论　该研究结果提示利用腺病毒载体携带IFN β基因来开发瘤苗治疗肿瘤具有良好的应用前景。更多还原

【Abstract】 AIM To observe the tumorigenicity and anti-tumor effects of adenovirus-mIFN-β transfected murine melanoma cells B16. METHODS The B16 cells were transefcted by recombinant adenovirus carrying mIFN-β gene in vitro, then the tumorigenicity and anti-tumor effects of AdmIFN-β transfected B16 cells in vivo was observed.RESULTS mIFN-β gene was successfully transduced into B16 cells mediated by adenovirus and expressed effectively. mIFN-β transduced into B16 cells mediated by adenovirus not only resulted in an efficient inhibition of tumorigenicity in situ but also led to a growth inhibition of parental cells at other sites. CONCLUSIONS This study indicates that it has bright prospect to develop tumor vaccine and treat tumor by using adenovirus vector harboring IFN-β gene.更多还原