【摘要】 目的　研究中华芦荟多糖 (AP)对X射线照射后人非瘤细胞株CLiver和 2 93细胞凋亡及细胞凋亡蛋白 p5 3,bax ,Bcl 2 ,Bad ,survivin表达的影响。方法　流式细胞仪检测细胞凋亡 ,Westernblot检测细胞凋亡相关蛋白的表达。结果　X射线照射后 72h ,CLiver细胞可见典型的DNA梯形条带 ,照射前加入AP 2 5或 5 0mg·L-1均能使DNA片段减少 ;AP预处理使CLiver细胞在照后 6h和72h凋亡率分别由 5 0 %和 4 3 0 %降至 2 2 %和 10 9% ,使2 93细胞照后 6h凋亡率由 8 6 %降至 4 0 % ;AP预处理可使CLiver细胞p5 3、Bad、Bax蛋白表达量下调 ,Bcl 2和sur vivin蛋白表达则上调。结论　AP对X射线辐射后非瘤细胞凋亡的抑制作用与对凋亡蛋白表达的调节有关更多还原

【Abstract】 AIM To study the modulation of Aloe Polysaccharides on the apoptosis and apoptotic regulating protein expression in X-ray irradiated non-malignant cells. METHODS The apoptosis was analyzed using flow cytometry. The level of apoptotic regulating protein expression was tested using Western blot. RESULTS A distinct DNA ladder happened in C Liver cells 72 h after irradiation. Pre-treatment of AP in the concentration of 25 or 50 mg·L -1 significantly decreased the DNA ladder. The apoptotic rate of C Liver in 6 h and 72 h after irradiation was decreased from 5.0%, 43.0% to 2.2% and 10.9%, respectively. Meanwhile, pre-treatment of AP down-regulated the expression of p53, Bad and Bax protein caused by irradiation and evidently up-regulated the expression of Bcl-2 and Survivin protein. On the other hand, The apoptotic rate of 293 cells in 6h after irradiation was decreased from 8.6% to 4.0%. CONCLUSION The inhibiting effect of AP on the apoptosis of non-malignant cells was at least partly caused by the modulation of apoptotic regulating protein expression.更多还原