【摘要】 目的　研究紫草素诱导HeLa细胞凋亡的作用机制。方法　MTT法测定紫草素对HeLa细胞的生长抑制实验 ,荧光染色等观察细胞形态学变化 ;DNA电泳、LDH法研究细胞死亡的途径。免疫印迹实验检测相关蛋白的表达。结果　紫草素具有明显的抑制HeLa细胞生长的作用 ,并呈明显的量效关系和时间依赖性 ,4 0 μmol·L- 1 的紫草素在 2 4h内可诱导细胞凋亡 ,凋亡途经与死亡受体信号途径相似 ,经历了半胱氨酸天冬氨酸蛋白酶 3和 8(caspase 3和caspase 8)的激活。结论　 4 0 μmol·L- 1 的紫草素可明显地诱导HeLa细胞凋亡 ,其信号转导途径为经典的caspase途径更多还原

【Abstract】 AIM To study the mechanisms of shikonin-induced HeLa cell apoptosis. METHODS Cytotoxicity assay by MTT, DNA fragmentation assay, LDH activity-based cytotoxicity assay and Western blot analysis were carried out. RESULTS Shikonin inhibited HeLa cell growth in a dose- and time-dependent manner. Apoptotic cell death was observed within 24 h after 40 μmol·L -1 shikonin treatment. Shikonin induced HeLa cell apoptosis involved in the activation of caspase-3 and caspase-8, which is similar to the Fas or TNF-α signaling transduction pathways. CONCLUSION Shikonin induced caspase-dependent apoptosis in HeLa cells.更多还原