【摘要】 目的　研究两种儿茶素ECG、EGCG对耐药人口腔表皮样癌细胞KBV2 0 0的促凋亡作用。方法　MTT法检测药物对细胞的毒性作用 ,用形态学观察法、TUNUL法、琼脂糖凝胶电泳及流式细胞仪PI染色法观察细胞凋亡。结果　 30mg·L-1ECG或 8mg·L-1EGCG联合 0 0 2 4mg·L-1长春新碱 (VCR)可用形态学观察、免疫组化法、琼脂糖凝胶电泳法见到细胞凋亡改变 ,而对照组无此类改变或改变不明显。结论　两种儿茶素在低细胞毒剂量时可促进长春新碱对耐药口腔表皮样癌细胞KBV2 0 0的细胞凋亡作用。更多还原

【Abstract】 AIM To study the apoptosis of KBV200, human oral carcinoma cells, administrated with ECG and EGCG. METHODS MTT was used to test the cytotoxicity of drugs. The apoptosis of KBV200 was observed by morphological method, TUNUL,an immunohistochemical method, DNA gel electrophoresis in agarose and FACS with PI-staining. RESULTS The apoptosis of KBV200 could be observed as KBV200 was administrated with 30 mg·L -1 ECG or 8～14 mg·L -1 EGCG combined with 0.024～0.05 mg·L -1 VCR by those methods above, while control group without combination treatment with slight apoptosis or no apoptosis observed. CONCLUSION ECG and EGCG, with low dose of cytotoxicity, could enhance the apoptosis of KBV200 induced by VCR.-更多还原