【摘要】 目的 :建立高效液相色谱法 (HPLC)测定西洛他唑在健康人体内的药动学参数。方法 :血浆样品以地西泮作内标 ,经乙醚提取并浓缩进样 ;采用HYPERSILC18分析柱 (2 0 0mm× 5 .0mm ,5 μm) ,以乙腈 磷酸盐缓冲液 (5 0∶5 0 )为流动相 ,流速 1.0mL·min- 1;柱温 2 5℃ ;在紫外 2 5 7nm处检测。结果 :标准曲线线性范围为 0 .0 2 73～ 3.4 880mg·L- 1,r=0 .9994 ;方法回收率 91.74 %～ 110 .0 9% ;日内RSD为 1.0 2 %～ 7.4 2 % ,日间RSD为 5 .0 8%～6 .85 %。结论 :HPLC测定西洛他唑血药浓度灵敏、可靠 ,回收率高 ,结果准确 ,能满足西洛他唑临床药动学和生物等效性研究更多还原

【Abstract】 AIM: To establish a high performance liquid chromatography （HPLC） method to determine the pharmacokinetic of cilostazol in healthy volunteers. METHODS: The drug and internal standard （diazepam） were extracted from plasma with ether. The compounds were separated on a HYPERSIL C₁₈ column （200 mm×5.0 mm, 5 μm ）. The mobile phase was composed of acetonitrile∶0.01 moL·L^-1 KH₂PO₄（50∶50）, the flow rate was 1.0 mL·min^-1. The column temperature was 25 ℃. The detection wavelength was UV 257 nm. RESULTS: A good linearity was obtained from 0.0273 to 3.4880 mg·L^-1 in plasma with a correlation coefficient of 0.9994. The recovery of method were from 91.74 to 110.09 %. The intra-day and inter-day RSD were （1.02～7.42） % and （5.08～（6.85） %,） respectively. CONCLUSION: The HPLC method is sensitive, a good recovery, accurate and reproducible and can be used for clinical pharmacokinetic and bioequialent study of cilostazol.更多还原