【摘要】 目的 :通过大鼠肝纤维化动物模型 ,研究抗肝纤方预防肝纤维化的作用 ,并探讨其作用机制。方法 :采用二甲基亚硝胺(DMN)诱导大鼠制备肝纤维化模型 ,以秋水仙碱为阳性对照 ,采用光镜观察组织学改变并测定血清谷丙氨酸转移酶 (ALT)、谷草氨酸转移酶 (AST)、白蛋白 (ALB)、球蛋白 (GLB)、透明质酸 (HA)、层粘连蛋白 (LN)、III型前胶原 (PCIII)、和IV型胶原(IV C) ,以反映肝细胞损伤及肝纤维化的程度。结果 :抗肝纤方能显著降低大鼠血清中ALT、AST、HA、LN、PCIII、IV C的水平 ,升高ALB。病理组织学检查亦表明 ,抗肝纤方能明显减轻实验大鼠肝脏纤维化程度。结论 :抗肝纤方对实验性肝纤维化有预防作用更多还原

【Abstract】 Objective: To investigate the effect of Kangganqian decoction in the Prevention of liver fibrosis through the rat model and to discuss its mechanism Method: The rat model of liver fibrosis was induced by intraperitoneal injection of DMN They were treated with Kangganqian decoction or colchicines as positive control The histological changes were observed with microscope The levels of ALT、AST、ALB、GLB、HA、LN、PCIII、IV C in serum were determined to reveal the event of liver damage Results: Kangganqian decoction significantly reduced the levels of ALT、AST、HA、LN、PCIII、IV C and improved the level of ALB in serum Pathological examination suggested with the degree of liver fibrosis was obviously relieved after the treatment of Kangganqian decoction Conclusion:Kangganqian decoction has preventive effect on liver fibrosis in the rats induced by DNM更多还原