【摘要】 目的 探讨鼻息肉中总IgE、IL-5的表达、嗜酸性粒细胞(eosinophils,EOS)浸润情况及其相关关系,以分析其在鼻息肉发病中的作用。方法 采用免疫组化SP法检测38例鼻息肉,15例正常鼻腔黏膜组织中总IgE阳性细胞分布情况;酶联免疫吸附试验(ELISA)检测鼻息肉中IL-5含量;Chromotrope 2R特染法检测鼻息肉中EOS浸润情况。结果 ①鼻息肉组织中总IgE、IL-5及EOS水平均明显高于正常鼻腔黏膜组织,P均<0.01;②鼻息肉组织中总IgE与EOS、IL-5与EOS以及IL-5与总IgE之间均呈明显正相关关系,相关系数r分别为0.843,0.642,0.667,P均<0.01。结论 EOS浸润在鼻息肉的形成和发展中起重要作用,而IgE及IL-5则对鼻息肉中EOS浸润发挥重要影响。更多还原

【Abstract】 Objective To investigate the correlation of total IgE and IL-5 contents and theinfiltration of eosinophils (EOS) in nasal polyps. Methods 38 patients with nasal polyps and 15samples of normal nasal mucosa were included in this study. For these cases, IL-5 content in thetissue homogenate of polyps was measured by ELISA,while the distribution of IgE-positive cellswas detected by SP immunohistochemical method and the infiltration of EOS was determined byChromotrop 2R histochemical method in the tissues of polyps and nasal mucosa. Results Thelevels of total IgE, IL-5 and EOS were all significantly higher in nasal polyps than that in normalnasal mucosa (P<0. 01), with very high positive correlation coefficients for IgE with EOS (0.843), IL-5 with EOS(0. 642) and IL-5 with IgE (0.667) respectively. Conclusion The infiltra-tion of EOS plays an important role in the pathogenesis and development of nasal polyps. Fur-thermore, IgE and IL-5 may be contributed to the infiltration of EOS in polyp tissue at a high lev-el.更多还原