【摘要】 目的　建立一种反相液相色谱法用于新复方制剂中川芎嗪和阿司匹林含量的同时测定。方法　采用DiamonsilC18色谱柱 ,以甲醇 -1 5%醋酸 (3 5∶65,V V ,pH =3 1)为流动相 ,流速为 1 0mL·min- 1。结果　色谱测定可在 12min内完成。苯甲酸为内标。在标示量 80 -12 0 %的范围内 ,川芎嗪和阿司匹林的回收率范围分别为 99 6-10 0 3 %和 99 9-10 1 3 %。川芎嗪和阿司匹林的线性范围分别为 2 5 0 -3 0 0 0 μg·mL- 1(r=0 9999,n =5)和 12 5-150 9μg·mL- 1(r=0 9997,n =5)。结论　本法可用于复方制剂中川芎嗪和阿司匹林含量的同时测定更多还原

【Abstract】 AimTo establish a reversed-phase liquid chr om atographic (LC) method for simultaneous determination of tetramethylpyrazine (TM P) and aspirin in a new compound formulation. MethodsChroma tographic separation of the two drugs was achieved on a Diamonsil C 18 colu mn, using a binary mixture of methanol-1.5% acetic acid (35∶65, V/V, pH=3 1 ) as mobile phase at a flow rate of 1 0 mL·min -1 . ResultsSeparation was completed in less than 12 min. Benzoic acid was used as the internal standard. Recoveries at levels corresponding to 80 % to 120 % of the l abel claim of the formulation ranged from 99 6 to 100 3 % for aspirin and from 99 9 to 101 3% for TMP. The linear range was 12 6-150 9 μg·mL -1 (r= 0 9997, n=5) for aspirin and 25 0-300 0 μg·mL -1 (r=0 9999, n =5) for TMP. ConclusionThe method developed can be used for the simult aneous determination of TMP and aspirin in pharmaceutical preparations.更多还原