【摘要】 目的　合成异核苷掺入寡核苷酸并考察它们与互补序列的结合能力。方法　采用DNA固相合成仪合成寡核苷酸 ,通过热变性实验考察双链的稳定性。结果　合成了四条单异核苷掺入的寡核苷酸 ,热变性实验结果发现异核苷的引入降低了双链的稳定性 ,当异核苷处于寡核苷酸链的中央时 ,这种影响更为明显。异核苷 6′ OH处于游离和烯丙基保护状态时的结果没有显著差异。结论　寡核苷酸中的异核苷使链发生扭曲 ,从而导致双链稳定性降低。更多还原

【Abstract】 AimTo synthesize isonucleoside-incorporated ol igonucleotides and investigate their binding abilities with complementary sequen ces. MethodsThe synthesis was performed on DNA synthesizer, and the binding behavior was investigated by thermal denaturation studies. ResultsFour kinds of single isonucleoside containing oligonucleoti des were synthesized. The results of thermal denaturation showed that the existe nce of isonucleoside decreased the stability of duplex, and the effect was more obvious when the isonucleoside was in the middle of the sequence. No obvious dif ference was observed when 6′-OH of isonucleoside was free or was protected by allyl group. ConclusionsThe existence of isonucleoside in oli gonucleotide makes chain twist and decreased the stability of duplex.更多还原