【摘要】 目的观察天然冰片对大鼠下丘脑单胺类神经递质NE、E、5-HT、DA含量的影响,同时进行其时效和量效关系的观察,初步探讨冰片的作用靶点和作用机理。方法将168只Wistar大鼠随机分组,分别灌服生理盐水、1%CMC-Na,以及高、中、低剂量的冰片,每天灌胃2次,在0、45min、24h、72h、120h不同时相,用高效液相-电化学法检测大鼠下丘脑单胺类神经递质NE、E、5-HT、DA含量。结果灌服冰片45min后,冰片中、高剂量组对下丘脑NE含量有显著降低作用(P<0.05);对下丘脑E的含量,中、高剂量组和CMC-Na组相比有明显的降低作用(P<0.05);连续灌胃后,不同时相冰片各组下丘脑NE、E含量和CMC-Na组相比,继续呈现不同程度的下降作用,但对下丘脑5-HT无显著影响;在连续灌胃5d后,对DA含量有显著降低作用(P<0.05)。对大脑皮层乙酰胆碱含量无显著影响,对单胺氧化酶含量有升高的趋势,但除在24h时相,冰片中、高剂量组差异有显著性意义外(P<0.05),其余时相无显著差异。结论冰片在各个时相均有降低下丘脑NE、E含量的作用,而且随着剂量的递增,对下丘脑中的NE、E含量有逐渐降低的趋势,表现出一定的量效关系。推测芳香开窍中药冰片的药理作用方式可能通过调节中枢神经组织中单胺类神经递质含量达到稳态平衡有关。更多还原

【Abstract】 Objective To investigate the effect of borneol on neurotransmitter in rats hypothalamus, to study the relations of dosage-effect and time-effect, for probing into the possible functionary mechanisms and target of borneol. Methods 168 Wistar rats were randomly divided into 5 groups, given 0.9% NS, 1% CMC-Na, and different dosages of nature borneol two times every day. At 0、45min、24h、72h、120h, monoamine neurotransmitter levels in rats hypothalamus were measured by HPLC combined with electrochemical detector method. Results In hypothalamus, comparing with 1% CMC-Na group, we find that the middle dosage and high dosage borneol can decrease markedly the NE and E levels in hypothalamus after giving borneol 45 minutes (P<0.05). And continuously giving borneol, the E and NE contents in hypothalamus is reduced too. After giving borneol, the 5-HT contents in hypothalamus, comparing with 1% CMC-Na group, the effect has not been found. And DA contents just after giving borneol five days, the middle and high dosage can markedly decrease the DA levels in hypothalamus. Conclusion Borneol can regulate the contents of monoamine neurotransmitter in hypothalamus.更多还原