【摘要】 目的探讨护肾固精方对肾小球系膜细胞增殖的影响及其作用机理。方法采用细胞培养与细胞分子学技术相结合方法,在大鼠肾小球系膜细胞培养体系中加入护肾固精方,分别以噻唑蓝法(MTT)和酶联免疫吸附法(ELISA),研究其对系膜细胞(Mesangialcell,MC)增殖和白细胞介素-8(IL-8)的影响。结果护肾固精方加入MC培养体系6d后,MC数(活细胞个数)为56835±6638,明显低于对照组138653±14388(P<0.01)。该方有抑制MC增殖的作用,而且这种抑制作用呈现一定量效关系,即药物浓度越高,抑制作用越明显。MC上清中IL-8含量对照组为0.168mg/L±0.113mg/L,治疗组为0.062mg/L±0.015mg/L,两组比较,有非常显著性差异(P<0.01)。结论护肾固精方有明显抑制肾小球MC增殖的作用。这种作用可能与其拮抗MC分泌IL-8等其它炎症介质有关。更多还原

【Abstract】 Objective To study the effects of the Hushengujing (HSGJ) prescription on the proliferation of the mesangial cell (MC) and its mechanism. Methods The proliferation of MC and the interleukocyte-8 (IL-8) in it were detected by MTT and ELISA respectively, after adding the medicine into the MC-cultivating system of SD rat. Results After adding the medicine to the MC-cultivating system 6 days, the MC decreased to 56835±6638 (live cell numbers), lower than the matched control with 138653±14388 (P<0.01). There was a certain quantity-effect relationship between the density of prescription and the inhibitory effect on the MC proliferation, namely the more high the medicine density, the more obvious its inhibiting effect. By detecting the MC pure liquid, IL-8 in the group with the HSGJ prescription (about 0.062mg/L±0.015mg/L) was lower than that in the matched control group (about 0.168mg/L±0.113mg/L) significantly (P<0.01). Conclusions The HSGJ prescription has an evidently inhibiting effect on the kidney MC proliferation. This effect might be attributed to the inflammatory medium secreted by MC.更多还原