【摘要】 目的　研究一氧化氮 (NO)对感染日本血吸虫小鼠早期肝脏纤维化的影响。方法　 NMRI小鼠感染日本血吸虫后第 2 3天起 ,连续用诱导型一氧化氮合酶 (i NOS)的抑制剂氨基胍(Am inoguanidine,AMG)处理小鼠 ,同时设未经抑制的对照组 ,分别在感染后第 38、4 5天观察小鼠肝脏的病理变化。天狼猩红染色 -偏振光显微镜观察并结合图像分析的方法 ,分析肝脏中 、 型胶原的动态变化 ;化学显色法检测肝脏匀浆中能间接反映肝脏纤维化程度的羟脯氨酸的浓度。结果　感染后 38d,肝脏中 、 型胶原的增生程度及 、 型胶原面积比值明显高于对照组 ,但羟脯氨酸的含量与对照组相比差异无显著性 (P>0 .0 5 ) ;感染后 4 5 d,肝脏中 型胶原的增生程度和 、 型胶原面积比值明显高于对照组 ,AMG处理组中羟脯氨酸的含量较对照组高 (P<0 .0 5 )。结论　 NO可抑制日本血吸虫感染小鼠肝脏早期纤维化更多还原

【Abstract】 Objective To investigate the effect of nitric oxide on the liver fibrosis of mice infected with Schistosoma japonicum during the early stage of schistosomiasis. Methods NMRI mice were treated with AMG-an iNOS inhibitor from day 23 post infection(p.i) to the sacrificed and the livers were collected at 38 days, 45 days p.i respectively. The expression and distribution of collagen typeⅠ, Ⅲ (ColⅠand ColⅢ) in liver tissues were investigated with the Picric acid-Sirius red staining techniques and differentiated with the polarization microscopy combining with picture analysis system. Hydroxyproline concentration in liver homogenate was measured by the biochemical methods. Results At 38 day p.i, the Picric acid-Sirius red staining showed that the hyperplasia of Col Ⅰ and ColⅢ in the livers of AMG treated mice increased significantly compared with the control livers, but there was no significant difference of hydroxyproline content between the two groups. At 45 days p.i, only the hyperplasia of Col Ⅰ in AMG treated group increased significantly compared with the control livers, and moreover, content of hydroxyproline of the inhibited mice was significantly higher than that of the control mice (P<0.05). Conclusion NO can inhibit fibrosis of liver during the early stage of infection caused by Schistosoma japonicum in mice.更多还原